• br Materials and methods br Statistical analysis

  2018-11-08

  

  Materials and methods Statistical analysis Data are presented as mean±standard error of the mean (SEM) of at least three independent experiments. Comparisons were performed using a two-tail unpaired t-test or a one sample t-test (real time PCR). Results Discussion In this work we have i

• Cytoskeletal and rhythmic contraction and pulsatile laminar

  2018-11-08

  

  Cytoskeletal and rhythmic contraction, and pulsatile laminar shear stress, are mechanical forces that have been suggested to play a crucial role in heart development and growth (Taber, 2001; Zhu et al., 2014; Andrés-Delgado and Mercader, 2016). Cytoskeletal contraction is involved in several morphog

• Many studies have demonstrated that

  2018-11-08

  

  Many studies have demonstrated that GSCs isolated from glioblastomas display a capacity for multiple-lineage differentiation (Hemmati et al., 2003; Matsuda et al., 2012; Singh et al., 2003, 2004; Sunayama et al., 2010b). When differentiated, these Brefeldin A usually lose the capacity for long-term

• While stem cell based therapies hold

  2018-11-08

  

  While stem cell-based therapies hold great potential for the treatment of a wide array of medical conditions they are so novel that product characterization is particularly challenging. Despite considerable progress, the molecular regulatory mechanisms of self-renewal and lineage specification in th

• Hydrogel biocompatibility is improved when the

  2018-11-08

  

  Hydrogel biocompatibility is improved when the mechanical properties of the hydrogel are matched to the host tissue (Aurand et al., 2012a,b; Banerjee et al., 2009; Engler et al., 2006; Georges et al., 2006; Saha et al., 2008; Seidlits et al., 2010; Teixeira et al., 2009). Since tissue-matching is an

• Gene expression is regulated by complex coordinated processe

  2018-11-08

  

  Gene expression is regulated by complex coordinated processes, including chromatin remodeling, post-translational modifications of histones, and incorporation of non-allelic histone variants. The histone variant H2AZ constitutes only a few percents of the total H2A cellular pool (). However, H2AZ is

• One of the major challenges

  2018-11-08

  

  One of the major challenges in scaling up of hESC cultures is the effects of the mechanical stimulation generated by the hydrodynamic shear forces which is an inherent property of agitated cultures (Earls et al., 2013). Previous studies done with mouse embryonic stem cells have shown that fluid shea

• The underlying justification for such adipose derived

  2018-11-08

  

  The underlying justification for such adipose-derived procedures is the assumption that the cellular extract contains mesenchymal stem l-ascorbic acid (MSCs) — a type of stromal cell isolated from a wide variety of sources including bone marrow, fat, dental pulp and placental tissue and one of the

• Based on our results we propose a panel of

  2018-11-08

  

  Based on our results, we propose a panel of potential hiPSC protein markers from the above-mentioned 22 proteins (Supplementary Table 12). We intentionally used three previously characterized stem cell lines (hiPSC: iNC-01, SB5-MP1 and hESC-H9) in our proteomic study. The genomic methylation profile

• br Design of donor plasmids If no

  2018-11-08

  

  Design of donor plasmids If no footprintless gene targeting is required or it is intended to introduce larger transgenes, donor plasmids have to be constructed that contain the homology arms flanking the transgenes or selection cassettes coupled to promoters of choice. Similarly, donor plasmids c

• Recently human umbilical cord hUC has been investigated

  2018-11-08

  

  Recently, human umbilical cord (hUC) has been investigated as a source of TAPI-1 with stem/progenitor cell properties. hUC is a desirable source of stem/progenitor cells as it is routinely discarded after delivery, collection is non-invasive and an abundant number of cells are present in cord tissue

• cholecystokinin receptor Accordingly we used the HC antibody

  2018-11-08

  

  Accordingly, we used the HC7 antibody to analyze the ACD behavior of defined CD133+ HSPC subpopulations. By tracking cholecystokinin receptor of the MPP fraction, almost 70% of them were found to perform ACDs in vitro, independent of whether they were tracked in bulk or single-cell cultures. In con

• microRNAs miRNAs are small noncoding RNAs

  2018-11-08

  

  microRNAs (miRNAs) are small noncoding RNAs of 22 nucleotides that control gene expression chiefly by translational repression (Lee et al., 2004). miRNA expression is spatially and temporally regulated during development, and they can participate in the regulation of pluripotent stem cell lineage co

• Despite the unmet medical need to date no

  2018-11-08

  

  Despite the unmet medical need, to date, no studies have demonstrated the feasibility of isolating ventricular CMs without permanently altering their genome. Prior studies used genetic modification for isolating ventricular CMs by inserting a fluorescent reporter gene driven by the MYL2 (or MLC-2v)

• br Experimental Procedures br Introduction Cells have been r

  2018-11-08

  

  Experimental Procedures Introduction β Cells have been reported to have the potential to proliferate after experimental ablation, such as chemical treatment or partial pancreatectomy (Bonner-Weir et al., 2010) (Cano et al., 2008; Nir et al., 2007; Xu et al., 2008). Although controversial, β d-

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