• Numerous published reports have examined the potential of

  2018-11-06

  

  Numerous published reports have examined the potential of differentiated cell types derived from mouse and human ESCs to repair nonhuman target organs in intact animals. Recent studies, however, have yielded both encouragement and caution, with restoration of function evident to some degree in many

• The use of a novel Pax Venus

  2018-11-06

  

  The use of a novel Pax9Venus reporter PSC line allowed the purification of PSC-derived Pax9+ AFE for molecular analysis and enabled the comparison to embryonic Pax9+ AFE. The expression of several pharyngeal endoderm transcription factors in PSC-derived Pax9+ AFE suggests that these EZ Cap Reagent A

• hydroxycarboxylic acid receptors Chondrogenic differentiatio

  2018-11-06

  

  Chondrogenic differentiation in our model is affected by deregulation of LMNA, especially in PG-MSCs, as revealed by a lowered COL2/COL1 ratio and lower expression of COL2 and AGG as shown by real-time RT-PCR. While our findings do not completely agree with the results of the study by Scaffidi and M

• To test the efficiency of gene knockdown the

  2018-11-06

  

  To test the efficiency of gene knockdown, the ES cell lines expressing the shPU.1 constructs were differentiated into hematopoietic cells. The transcription factor PU.1 is not expressed in ES g protein coupled receptors at day 0 of differentiation and becomes expressed by day 9 when hematopoietic p

• br Acknowledgments br Introduction Two developmental

  2018-11-06

  

  Acknowledgments Introduction Two developmental processes take place during pre-implantation in the development of mammalian embryos. The outer ubiquitin e3 ligase of the morula differentiate into a trophoectoderm (TE) lineage, whereas the inside cells become the inner cell mass (ICM). Further

• orexin The FRGN strain developed herein is suitable for

  2018-11-06

  

  The FRGN strain developed herein is suitable for hematopoietic reconstitution and in addition to this has some other advantages over our originally reported FRG KO on the C57BL6 strain (Azuma et al., 2007). While the final percentages of highly reconstituted (>80%) mice did not differ significantly

• br Materials and methods br Results br Discussion

  2018-11-06

  

  Materials and methods Results Discussion Strategies aiming at controlled divergence of ASCs to specific differentiated cell types essentially rely on prolonged culture in the presence of specific sets of growth factors and/or matrix proteins (Zuk et al., 2001). Specific culture conditions w

• In contrast to the variable descriptions of heart regenerati

  2018-11-06

  

  In shk to the variable descriptions of heart regeneration in amphibians, zebrafish can regenerate following multiple types of cardiac injury including ventricular resection, cryoinjury and genetic cardiomyocyte ablation (Gonzalez-Rosa et al., 2011; Poss et al., 2002; Wang et al., 2011). Cardiac inj

• The intestinal epithelium is a particularly attractive biolo

  2018-11-06

  

  The intestinal epithelium is a particularly attractive biological adult model to study epigenetic mechanisms driving differentiation in vivo. In the intestinal epithelium, two populations of SC have already been described and isolated (Lgr5+ and Bmi1+ cells; Barker et al., 2007; Sangiorgi & Capecchi

• br DNA fingerprinting analysis The

  2018-11-06

  

  DNA fingerprinting analysis The target regions of genomic DNA were amplified by Polymerase chain reaction (PCR). The PCR was carried out using a Thermocycler type PTC100 (MJ Research Inc) for 35cycles and each cycle contained 96°C for 30s, 56°C for 30s and 72°C for 30s. The following primer seque

• br Introduction The grafting of

  2018-11-06

  

  Introduction The grafting of cultured keratinocytes to promote regeneration represents one of the oldest clinical examples of stem cell therapy (Green, 2008). The skin constitutes an essential barrier between the living tissues of the body and the external environment, and skin tissues have evolv

• We next assessed how reflective the HDR editing

  2018-11-05

  

  We next assessed how reflective the HDR editing rates calculated via deep-sequencing were of actual editing rates. Hence, the CTS protocol was repeated for all seven variants independently, and approximately 250 clonal populations were then picked and analyzed via Sanger sequencing. We successfully

• Due to the poor regenerative capacity of the CNS there

  2018-11-05

  

  Due to the poor regenerative capacity of the CNS, there are few treatment options for SCI, and these options yield only modest clinical benefits at best. However, emerging data suggest that cell transplantation (TP) therapies represent a potential therapeutic intervention for SCI. We and others have

• Detailed description of the process of NPC

  2018-11-05

  

  Detailed description of the process of NPC samples fabrication from the carbides of boron, zircon, titanium and molybdenum and typical parameters of the product structure were disclosed in [53–55]. The structural parameters substantially differed for the samples of different origin. Thus, the mean p

• Ro 31-8220 methanesulfonate manufacturer The following formu

  2018-11-05

  

  The following formula was used to assess the values of the magnetic moments in both compounds: where μ (in Bohr magnetons μB) is the magnetic moment; M is the magnetization; B (in Oe) is the applied measuring magnetic field; kB is the Boltzmann constant, N is the number of magnetic atoms per unit o

16368 records 1075/1092 page Previous Next First page 上5页 10711072107310741075 下5页 Last page